Rapidase protocols

Rapidase® Revelation Aroma is a high-purity glycosidase preparation engineered to act on the full range of glycosidically bound aroma precursors.
It is used in two distinct applications:

Aroma release in any wine (terpenes, norisoprenoids)
Smoke taint management, as the first step of smoke taint remediation by hydrolysing bound smoky compounds

Below are the operational protocols for each application.

A) Aroma release protocol

Objective

Evaluate the aromatic impact of Rapidase Revelation Aroma through a controlled 6-week bottle trial.

Material:

7 bottles of 750 mL
Rapidase Revelation Aroma enzyme

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1. Prepare 7 × 750 mL bottles

Use the same wine for all bottles.
One bottle is kept as the untreated control (no enzyme added).
The remaining six bottles will receive weekly enzyme additions.

2. Prepare the enzyme solution (to achieve 2 g/hL)

For the test to remain representative, the enzyme solution is prepared in wine, not water, to avoid dilution.

Preparation:

• Dissolve 0.5 g of Rapidase Revelation Aroma in 500 mL of the wine being tested.
• Prepare a fresh solution every week.
• Each week, use 15 mL of this fresh solution per bottle.

3. Weekly enzyme addition (6 weeks)

For each of the six treatment bottles:

Add 15 mL of the freshly prepared enzyme solution.
Close the bottle immediately.
Store all bottles away from light, at cellar temperature (>13°C).
Repeat once per week for six consecutive weeks.

Each bottle corresponds to one contact time: Week 1 → Week 6.

4. Tasting evaluation

Perform tasting one week after the last addition.

Evaluate for each bottle (control + weeks 1–6): colour, nose, palate, and observations.

Goal: identify when the aroma release reaches optimum expression relative to the control.

5. Reproduce at full scale

Once the preferred contact time is identified, apply 2 g/hL in the tank at cellar temperature, and after completion, inactivate with 5–10 g/hL bentonite.

B) Smoke taint reduction protocol

Principle

Smoke-related volatile phenols exist in wine as glycosides. Using a complete glycosidase formulation will make available all the free forms of glucosides, which can then be removed.